• Dr. Emile van den Akker, dr. Robin van Bruggen, and dr. Marieke von Lindern (Sanquin, Amsterdam, The Netherlands)

• Dr. Kees Harteveld (Leiden University Medical Center, Leiden, The Netherlands)

• Dr. Marije Bartels, dr. Minke Rab, dr. Richard van Wijk (University Medical Center Utrecht, Utrecht, The Netherlands)

• 07:30 – 08:45

  Breakfast

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Scientific session 1 – Erythropoiesies

Chairs:  Wassim el Nemer / Catarina Freire 

• 09:00 – 09:30

  Erythromyeloblastic Islands
  Lionel Blanc, Northwell, United States

• 09:30 – 09:45

  Anemia links β-catenin with FOXO3 signaling during erythroid terminal differentiation
  Santhe van der Meulen, Sanquin, the Netherlands

• 09:45 – 10:00

  In vitro culture of erythroblasts (EBL) and production of mature erythrocytes for transfusions requires upscaling in fluidic-turbulent systems
  Giulia Iacono, Sanquin, the Netherlands

• 10:00 – 10:15

  The Role of the Zcchc6/Dis3L2 RNA Editor-Exonuclease Axis in Terminal Erythroid Differentiation
  Areum Han, Harvard Medical school / Boston’s Children Hospital, United States

• 10:15 – 10:30

  Transfusion-ready red blood cell generation from induced pluripoten stem cells (iPSCs)
  Emile van den Akker, Sanquin, the Netherlands

• 10:30 – 10:45

  Detailed characterization of iPSC-derived hematopoiesis and erythropoiesis en self-organized cellular complexes (hemanoids)
  Isabel Dorn, Medical University Graz, Austria

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• 10:45 – 11:15

  Coffee break

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Scientific session 2 – Ion channels of the red blood cell – function and disorders

Chairs: Giampaolo Minetti /  Silvia Neri

• 11:15 – 11:45

  Defects of RBC cell volume regulation and hydration state: their pathophysiology and diagnosis
  Paola Bianchi, Fondazione IRCCS Ca’ Granda Policlinico Milan, Italy

• 11:45 – 12:00

  Electrophysiological characterization of red blood cells using the automated patch clamp
  Nicoletta Muarciano, Nanion Technologies, Germany

• 12:00 – 12:15

  Role of Piezo 1 in terminal density reversal of healthy red blood cells
  Kuntal Dey, University of Zurich, Switserland

• 12:15 – 12:30

  Red Blood Cell Protein contribution to Malaria Parasite Invasion. Basigin mediation of Plasmodium falciparum red blood cell invasion does not require its transmembrane domain or interaction with monocarboxylate transporter
  Timothy Satchwell, University Bristol, UK
  

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• 12:30

  Lunch
  

• Networking session

• Poster presentations – titles and authors can be found below this program

• ‘Meet the expert’ session with Ingolf Bernhardt
  Unsolved problems in red cell research
  Ingolf Bernhardt, University Saarland, Germany

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Scientific session 3 – Red blood cell membrane and associated disorders  & 4. Clinical hematology – new treatments in sickle cell disease and other hereditary red blood cell disorders

Chairs: Stephane Egee / Min Qiao

Red blood cell membrane and associated disorders

• 16:00 – 16:30

  Membranopathies : from cytology and red cell/reticulocyte indices to molecular diagnosis, including prenatal diagnosis
  Lydie da Costa, University Paris, France

• 16:30 – 16:45

  Developmental changes in membrane lipid composition during terminal maturation of circulating human reticulocytes and ageing of red cells
  Giampaolo Minetti, University of Pavia, Italy

• 16:45 – 17:00

  Modulation of terminal erythropoiesis by a TSPO ligand
  Alice Dussouchaud, Inserm, France

• 17:00 – 17:15

  Complete absence of GLUT1 does not impair human terminal erythroid differentiation
  Catarina Freire, University of Bristol, United Kingdom

• 17:15 – 17:30

  Dual action of Dooku1 on PIEZO1 channel in human red blood cells
  Guillaume Bouyer, CNRS, France

Clinical hematology – new treatments in sickle cell disease and other hereditary red blood cell disorders

• 17:30 – 17:45

  Single center report of hemoglobinopathy free survival after pediatric hematopoietic stem cell transplantation
  Gertjan Lugthart, pediatrician, LUMC, Leiden, the Netherlands

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• 18:45 – 22:00

  Dinner

• 07:30 – 08:45

  • Breakfast

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Scientific session 6 – New developments for red cell research  and diagnostics

Chairs: Paola Bianchi / Jonathan de Wilde

• 09:00 – 09:30

  Probing red blood cells’s bio-mechanical properties
  Lars Kaestner, University of Saarland, Germany

• 09:30 – 09:45

  An Efficient and Specific Reporter System Suitable for High-Throughput Screening of Novel Fetal Hemoglobin Inducers
  Tingyue Li, ErasmusMC,  Rotterdam, the Netherlands

• 09:45 – 10:00

  Pyruvate Kinase Thermostability Is Associated with Red Blood Cell Adhesion, Deformability and Oxygen Affinity in Patients with Sickle Cell Disease
  Marissa Traets, UMC Utrecht, the Netherlands

• 10:00 – 10:15

  Towards quantification of intracellular free calcium in individual red blood cells by fluorescence lifetime imaging microscopy
  Min Qiao, Saarland University, Germany

• 10:15 – 10:30

  Ex vivo glycolytic flux measurements in red blood cells
  Titine Ruiter, UMC Utrecht, the Netherlands

• 10:30 – 10:45

  Hepatic Leukemia Factor as a marker for iPSC – derived Hematopoietic Stem Cells
  Maria Simanovich, Erasmus MC Rotterdam, the Netherlands

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• 10:45 – 11:15

  Coffee break

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Scientific session 7 – New developments for red cell research and diagnostics & 6. New developments for red cell research and diagnostics

Chairs: Lars Kaestner / Giulia Iacono

Clinical cases

• 11:15 – 11:45

  SUPT5H nonsense variants as modifying factor in beta-thalassemia trait
  Kees Harteveld, Leiden University MC, The Netherlands

New developments for red cell research and diagnostics

• 11:45 – 12:00

  An AI-based imaging flow cytometry approach to study erythrophagocytosis
  Silvia Neri, Sanquin, the Netherlands

• 12:00 – 12:15

  Endothelialized 3D perfusion model to study ex vivo reticulocyte-to-erythrocyte transition
  Claudia Maria Bernecker, University of Graz, Austria

• 12:15 – 12:30

  Actomyosin-driven expulsion of hemoglobin during hemolysis,
  Tessa Korsa, Fraunhofer IBMT, Sulzbach, Germany

• 12:30 – 12:45

  Red cell function devices under development for use in sickle cell disease
  Vivien Sheehan, Emory University School of Medicine, Atlanta, United States

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• 12:45 – 13:45

  Lunch

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Scientific session 8 – Clinical hematology – new diagnostic approaches & 2. Ion Channels

Chairs: Vivian Sheehan / Myrthe van Dijk

• 13:45 – 14:15

  Biomarkers in sickle cell disease
  Minke Rab, UMC Utrecht, The Netherlands

• 14:15 – 14:30

  Novel direct plasma heme-related assay decyphers hemolytic anemia and ineffective erythropoiesis
  Nicolas Hebert, Etablissement Francais Du Sang IDF, France

• 14:30 – 14:45

  Impact of Sickle Cell Disease Patients-Derived Red Blood Cells on Clot Dynamics and Fibrin Structure
  Naoual Ouazzani Chahdi, Sanquin, the Netherlands
  

• 14:45 – 15:00

  Low Voxelotor Concentrations Exacerbate Sickle Cell Formation At Low Oxygen Pressure
  Emile van den Akker, Sanquin, the Netherlands

• 15:00 – 15:15

  Soluble Transferrin Receptor as a Marker for Assessing Disease Severity in  Hereditary Hemolytic Anemias
  Geoffrey Kuppens, UMC Utrecht, the Netherlands
  Presented by Marije Bartels, UMC Utrecht, the Netherlands

• 15:15 – 15:30

  Autoimmune hemolytic anemia anti-RBC IgM, but not IgG, drive in vitro complement activation
  Femke Mulder, Sanquin, the Netherlands

Ion Channels

• 15:30 – 15:45

  Ion channels of the red blood cell:
  Effects of Pyruvate Kinase Activation in Hereditary Hemolytic Anemia
  Jonathan de Wilde, UMC Utrecht, the Netherlands

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• 15:45 – 16:15

  Coffee break, followed by sessions:
  

• 15:45 – 16:45

  Poster presentations – titles and authors can be found under this program

• 15:45 – 17:00

  Steering Committee meeting on the consensus-based Recommendation for the Diagnosis of Rare Hemolytic Anemia
  Sponsored by EuroBloodNet

• 17:00 – 17:45

  ERCS General Assembly meeting 

• 18:30 – 21:30

  Dinner

• 07:00 – 08:00

  Breakfast and checkout, payment of expenses (bar)

• 08:30

  Departure from hotel by bus to harbor

• 09:00

  Departure by ferry ‘Brakzand’ to mainland

Posters, format A0, portrait

• Pseudo Action Potentials (PAPs) in red blood cells – an approach of understanding
  Lars Kaestner, University of Saarland, Germany

• Anemia in aged mice is accompanied by reduced erythroid lineage cell production and increased red blood cell life span
  Carsten Alt, Pfizer

• A novel allosteric activator of pyruvate kinase enhances human and mouse enzymatic activity and leads to hematological improvements in sickle cell disease mouse models
  Carsten Alt, Pfizer

• Can the emergence of cation channel activity during storage threaten the survival of transfused red blood cells?
  Stephane Egee, CNRS, France

• Cell Therapy Scale-up: Bioprocess Development for the Production of Hematopoietic Cells
  Brenda Juarez Garza, Delft University, the Netherlands

• The quality of red blood cells isolated from cord blood during storage
  Joyce Bestebroer, Sanquin, the Netherlands

• Variability of extracellular particle content in red blood cell concentrates upon storage is associated with differential membrane alterations and could be used as quality control
  Donatienne Tyteca, UC Louvain, Belgium

• Hemoglobin as a multifunctional sensor and buffer
  Anna Bogdanova, University of Zurich, Switzerland

• Scale-up of stem cell cultures from shake flask to bioreactor: a CFD-based comparison of hydrodynamic stress
  Ramon van Valderen, Delft University, the Netherlands

• Towards an improved erythroid production from IPSC
  Marien van der Stel, Sanquin, the Netherlands

• Investigating the Role of Shear Stress on Endothelial-to-Hematopoietic Transitioning
  Oyishee Ahmad, Sanquin, the Netherlands

• A clinical mechanical marker of red blood cell deformability: sickle cell disease example
  Cecile Jebane, CNRS-CINam, Marseille, France

• RBC delivered antigens for the induction of antigen specific immune tolerance,
  Youmna Al Halabi, Sanquin, the Netherlands